Protein-protein and protein-ligand interactions are major events in many critical biological pathways. My research focuses on investigating the molecular and structural basis of these interactions. To that end, I utilize molecular biology and protein biochemistry approaches to obtain functionally active recombinant proteins. With those, we reconstitute and monitor biological events in vitro to directly correlate protein structure-function with biological outcome. We utilize various bioanalytical and biophysical methodology including, but not limited to, CD, fluorescence, SEC-MALS, ITC, SPR, SWAX and HPLC as well as conventional biochemistry. We are currently studying HIV virulence factor-host protein interactions, Cullin-based E3 ubiquitin ligase assembly, regulation of tumor suppressor p53 and its homologs functions by repressors and activators.
Visit Jinwwo's lab website
Education & Training
Yonsei University, Seoul, Korea
B.S. 1991 in Chemistry
The Ohio State University
Ph.D., 1997 in Chemistry
Columbia University, New York
Postdoctoral Associate 1997 – 2000
Jung J, Byeon IJ, Delucia M, Koharudin LM, Ahn, J., Gronenborn AM. Binding of HIV-1 Vpr to the Human Homolog of the Yeast DNA Repair Protein RAD23 (hHR23A) Requires Its XPC Binding (XPCB) as Well as the Ubiquitin Associated 2 (UBA2) Domains. J. Biol. Chem. 289 2577-2588 (2014).
Mitra, M, Hercík, K, Byeon, IJ, Ahn, J., Hill, S, Hinchee-Rodriguez, K, Singer, D, Byeon, CH, Charlton, LM, Nam, G, Heidecker, G, Gronenborn, AM, Levin, JG. Structural determinants of human APOBEC3A enzymatic and nucleic acid binding properties. Nucleic Acids Res. 42, 1095-1110 (2014).
Han, Y, Hou, G, Suiter, CL, Ahn, J., Byeon, IJ, Lipton, AS, Burton, SD, Hung, I, Gor’kov, PL, Gan, Z, Brey, WW, Rice, D, Gronenborn, AM, Polenova, TE. Magic Angle Spinning NMR Reveals Sequence-Dependent Structural Plasticity, Dynamics, and the Spacer Peptide 1 Conformation in HIV-1 Capsid Protein Assemblies. J. Am. Chem. Soc. 135, 17793-17803 (2013).
Ji, X.#, Wu, Y.#, Yan, J.#, Mehrens, J., Yang, H., DeLucia, M., Hao, C., Gronenborn, A. M., Skowronski, J., Ahn, J.*, and Xiong, Y*. Mechanism of allosteric activation of SAMHD1 dGTP. Nat. Struct. Mol. Biol. Doi:10.1038/nsmb.2692, #Equal contribution, *Corresponding Authors.
DeLucia M., Mehrens, M., Wu, Y., and Ahn, J. HIV-2 and SIVmac accessory virulence factor Vpx down-regulates SAMHD1 catalysis prior to proteasomal-dependent degradation. J. Biol. Chem. 288, 19116-19126 (2013)
Pittsburgh Center for HIV Protein Interactions (PCHPI)
09/30/12 – 07/31/17
National Institutes of Health